Journal: Aging (Albany NY)

Article Title: Impact of cytotoxic agents or apoptosis stimulants on αklotho in MDCK, NRK-52E and HK2 kidney cells

doi: 10.18632/aging.204238

Figure Lengend Snippet: Selective PPARγ antagonist SR-202 blunts cisplatin-dependent αklotho gene expression in MDCK cells. Arithmetic mean ± SEM of αklotho transcripts relative to TBP in MDCK cells treated with 3 μM cisplatin or vehicle control in the absence (left bars) or presence (right bars) of 200 μM PPARγ antagonist SR-202 for 24 h ( n = 8, repeated measures ANOVA followed by Dunnett post hoc test). ** p < 0.01 indicates significant difference from vehicle control (1 st bar vs. 2 nd bar), # indicates significant difference from the absence of PPARγ inhibitor SR-202 (2 nd bar vs. 4 th bar); Abbreviations: a. u.: arbitrary units; ctr: control.

Article Snippet: Selective PPARγ inhibitor SR-202 (Biomol, Hamburg, Germany) was added to the culture medium along with cisplatin at 200 μM.

Techniques: Expressing